Procedure

Take a few freshly plucked green spinach leaves.
Using scissors, cut the spinach leaves into small pieces and let them fall into the mortar.
Take a measuring cylinder that contains 5ml of acetone and pour it into the mortar.
Grind the spinach leaves using the mortar and pestle.
Place the extract into a watch glass using a spatula.
Take a strip of filter paper having a narrow notch at one end of the strip.
Take a pencil and a scale and draw a horizontal line with a pencil about 2-3 cm away from the tip of the notch.
Put a drop of the pigment extract in the middle of the line with the help of a capillary tube.
Allow the drop to dry and repeat till four or five drops are placed on the paper.
Take the chromatographic chamber and pour ether acetone solvent in it.
Fold one end of the filter paper strip and staple it.
Using a thread, hang the filter paper strip in the chromatographic chamber.
The loading spot should remain about 1 cm above the solvent level.
Leave the chromatographic chamber undisturbed for some time.
We can observe, as the solvent moves through the paper, it spreads the different pigments of the mixture to various distances.
When the solvent rises about 3/4th up the strip, remove the strip carefully and let it dry.

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