XI Biology lab work- to test sugar in urine.

 

1.Benedict's Test

Materials required:

Test tube, test tube holder, urine sample, measuring cylinders, Benedict’s solution and burner.

Procedure:

• Take 2 ml urine sample in a measuring cylinder from the urine sample bottle.
• Take a test tube and pour the urine sample in it.
• Take 5 ml Benedict’s reagent in a measuring cylinder.
• Add Benedict’s reagent to the test tube that contains urine sample.
• Using a test tube holder, hold the test tube firmly and heat it for 2 minutes on the burner.
• Keep shaking the test tube while heating.
• A yellow precipitate appears which indicates the presence of sugar in urine.
• Depending upon the concentration of sugar in the urine, either green, yellow, or brick red precipitates are formed.

2.Fehling's test

Materials required

Test tube, test tube holder, urine sample, measuring cylinders, Fehling’s solution A, Fehling’s solution B and burner.

Procedure

• Take 2 ml urine sample in a measuring cylinder from the urine sample bottle.
• Take a test tube and pour the urine sample in it.
• Take 2 ml Fehling’s solution A in a measuring cylinder.
• Add Fehling’s solution A to the test tube that contains urine sample.
• Take 2 ml Fehling’s solution B in a measuring cylinder.
• Add Fehling’s solution B to the test tube that contains urine sample.
• Using a test tube holder, hold the test tube firmly and heat it gently for 2 minutes on the burner.
• Keep shaking the test tube while heating.
• A green precipitate appears which indicates the presence of traces of sugar in urine.
• Depending upon the concentration of sugar in the urine, either green, yellow or brick red precipitates are formed.